Actin polymerization state regulates osteogenic differentiation in human adipose-derived stem cells

Abstract Background Actin is an essential cellular protein that assembles into microfilaments and regulates numerous processes such as cell migration, maintenance of shape, material transport. Methods In this study, we explored the effect actin polymerization state on osteogenic differentiation human adipose-derived stem cells (hASCs). The hASCs were treated for 7 days with different concentrations (0, 1, 5, 10, 20, 50 nM) jasplakinolide (JAS), a reagent directly polymerizes F-actin. effects proliferation, apoptosis, maturity focal adhesion-related proteins assessed. addition, western blotting alizarin red staining assays performed to assess differentiation. Results Cell proliferation migration in JAS 20 groups higher than control group (50 group. FAK, vinculin, paxillin, talin expression levels highest (20 group, while zyxin was Western showed enhanced compared strongest Conclusions summary, our data suggest may promote by regulating adhesion-associated concentration-dependent manner. Our findings provide valuable information exploring mechanism hASCs.